PHOTOBLEACHING RECOVERY STUDIES OF MEMBRANE EVENTS ACCOMPANYING LECTIN STIMULATION OF RABBIT LYMPHOCYTES

被引:8
作者
LEUTHER, MD [1 ]
BARISAS, BG [1 ]
PEACOCK, JS [1 ]
KRAKAUER, H [1 ]
机构
[1] WASHINGTON STATE UNIV, DEPT BIOCHEM, PULLMAN, WA 99164 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0006-291X(79)90946-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fluorescence photobleaching recovery methods reveal marked changes in lateral mobilities of rabbit lymphocyte membrane components during the course of stimulation with succinyl concanavalin A (S Con A). The diffusion constant of S Con A receptors on T lymphocytes falls from 1.6×10-10 cm2/sec to 6.5×10-11 cm2/sec within 4 hr after stimulation, remains constant for 14 hr, and returns to its former value. The mobility of B cell receptors similarly falls from 1.4×10-10 cm2/sec to 5.5×10-11 cm2/sec but regains its unstimulated value much more slowly. In contrast, a fluorescent phospholipid analog shows constant mobilities of 1.9×10-8 cm2/sec and 1.5×10-8 cm2/sec in T and B cells, respectively, throughout the experiment. © 1979.
引用
收藏
页码:85 / 90
页数:6
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