PHOTOBLEACHING RECOVERY STUDIES OF MEMBRANE EVENTS ACCOMPANYING LECTIN STIMULATION OF RABBIT LYMPHOCYTES

被引：8

作者：

LEUTHER, MD ^{[1
]}

BARISAS, BG ^{[1
]}

PEACOCK, JS ^{[1
]}

KRAKAUER, H ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV, DEPT BIOCHEM, PULLMAN, WA 99164 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(79)90946-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence photobleaching recovery methods reveal marked changes in lateral mobilities of rabbit lymphocyte membrane components during the course of stimulation with succinyl concanavalin A (S Con A). The diffusion constant of S Con A receptors on T lymphocytes falls from 1.6×10-10 cm2/sec to 6.5×10-11 cm2/sec within 4 hr after stimulation, remains constant for 14 hr, and returns to its former value. The mobility of B cell receptors similarly falls from 1.4×10-10 cm2/sec to 5.5×10-11 cm2/sec but regains its unstimulated value much more slowly. In contrast, a fluorescent phospholipid analog shows constant mobilities of 1.9×10-8 cm2/sec and 1.5×10-8 cm2/sec in T and B cells, respectively, throughout the experiment. © 1979.

引用

页码：85 / 90

页数：6

共 13 条

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[12] LATERAL TRANSPORT ON CELL-MEMBRANES - MOBILITY OF CONCANAVALIN A RECEPTORS ON MYOBLASTS
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KOPPEL, DE
AXELROD, D
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[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (07) : 2409 - 2413
[13] STUDIES ON MECHANISM BY WHICH CYANINE DYES MEASURE MEMBRANE-POTENTIAL IN RED BLOOD-CELLS AND PHOSPHATIDYLCHOLINE VESICLES
SIMS, PJ
WAGGONER, AS
WANG, CH
HOFFMAN, JF
[J]. BIOCHEMISTRY, 1974, 13 (16) : 3315 - 3330

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