STRUCTURES OF ASPARAGINE-LINKED OLIGOSACCHARIDES FROM HEN EGG-YOLK ANTIBODY (IGY) - OCCURRENCE OF UNUSUAL GLUCOSYLATED OLIGO-MANNOSE TYPE OLIGOSACCHARIDES IN A MATURE GLYCOPROTEIN

被引:66
作者
OHTA, M
HAMAKO, J
YAMAMOTO, S
HATTA, H
KIM, M
YAMAMOTO, T
OKA, S
MIZUOCHI, T
MATSUURA, F
机构
[1] FUKUYAMA UNIV,FAC ENGN,DEPT BIOTECHNOL,FUKUYAMA,HIROSHIMA 72902,JAPAN
[2] FUJITA GAKUEN UNIV,SCH MED,INST COMPREHENS MED SCI,DIV BIOMED POLYMER SCI,TOYOAKE,AICHI 47011,JAPAN
[3] TAIYO KAGAKU CO LTD,CENT RES LABS,YOKKAICHI,MIE 510,JAPAN
[4] HIROSHIMA UNIV,FAC ENGN,DEPT FERMENTAT TECHNOL,HIROSHIMA 724,JAPAN
关键词
ASN-LINKED OLIGOSACCHARIDE; HEN EGG-YOLK; IGY; IMMUNOGLOBULIN; P-AMINOBENZOIC ACID ETHYL ESTER; HPLC; GLUCOSYLATED OLIGOSACCHARIDE;
D O I
10.1007/BF00731292
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Asparagine-linked oligosaccharides present on hen egg-yolk immunoglobulin, termed IgY, were liberated from the protein by hydrazinolysis. After N-acetylation, the oligosaccharides were labelled with a UV-absorbing compound, p-aminobenzoic acid ethyl ester (ABEE). The ABEE-derivatized oligosaccharides were fractionated by anion exchange, normal phase and reversed phase HPLC, and their structures were determined by a combination of sugar composition analysis, methylation analysis, negative ion FAB-MS, 500 MHz H-1-NMR and sequential exoglycosidase digestions. IgY contained monoglucosylated oligomannose type oligosaccharides with structures of Glc-alpha-1-3Man7-9-GlcNAc-GlcNAc, oligomannose type oligosaccharides with the size range of Man5-9GlcNAc-GlcNAc, and biantennary complex type oligosaccharides with core region structure of Man-alpha-1-6(+/- GlcNAc-beta-1-4)(Man-alpha-1-3)Man-beta-1-4(+/-Fuc-alpha-1-6)GlcNAc. The glucosylated oligosaccharides, Glc1Man8GlcNAc2 and Glc1Man7GlcNAc2, have not previously been reported in mature glycoproteins from any source.
引用
收藏
页码:400 / 413
页数:14
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