SITE DIRECTED MUTAGENESIS - A TOOL FOR ENZYME MECHANISM DISSECTION

被引：31

作者：

WAGNER, CR

BENKOVIC, SJ

机构：

[1] C.R. Wagner and S.J. Benkovic are, the Pennsylvania State University, Department of Chemistry, University Park

来源：

TRENDS IN BIOTECHNOLOGY | 1990年 / 8卷 / 09期

关键词：

D O I：

10.1016/0167-7799(90)90189-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Protein engineering has become the principle means of examining the active site of an enzyme to identify and quantify the roles of specific residues in ligand binding, specificity and catalysis. Site-specific mutagenesis has extended our knowledge gained from X-ray crystallography, and has provided striking proof that the intricate active-site geometry is supported by the remainder of the protein infrastructure for maximum catalytic efficiency. © 1990.

引用

页码：263 / 270

页数：8

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