EXPRESSION OF T-CELL RECEPTOR GENES IN THE THYMUS - LOCALIZATION OF TRANSCRIPTS INSITU AND COMPARISON OF MATURE AND IMMATURE SUBSETS

被引:16
作者
HELD, W
MUELLER, C
MACDONALD, HR
机构
[1] LUDWIG INST CANC RES, LAUSANNE BRANCH, CH-1066 EPALINGES, SWITZERLAND
[2] UNIV BERN, DEPT PATHOL, CH-3000 BERN, SWITZERLAND
关键词
D O I
10.1002/eji.1830200936
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have analyzed the expression of T cell receptor (TcR) genes in the thymus using in situ RNA hybridizations with probes to the constant regions of the TcR α, β, γ and δ chains. Localization of transcripts revealed low TcR α mRNA levels in the thymus cortex and very low levels in the subcapsular region. In contrast, TcR β message was very abundant in the cortex. TcR γ or δ mRNA+ thymocytes showed a scattered, predominantly cortical localization. In contrast to γ, TcR δ transcripts were abundant in the subcapsular region. Control experiments with sorted TcR α/β or γ/δ cells revealed a detection efficiency of 75%–85% for the respective TcR mRNA and data on TcR gene expression in mature, CD3+ thymocytes were consistent with previous reports. The analysis of immature, CD3− thymocyte subsets, however, revealed a virtual absence of TcR α transcripts and an unexpectedly high proportion of cells (14%–29%) expressing the gene for the TcR δ chain. The data are discussed in view of current models of lineage relationships in the thymus. Copyright © 1990 Wiley‐VCH Verlag GmbH & Co. KGaA
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页码:2133 / 2136
页数:4
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