EXPRESSION OF T-CELL RECEPTOR GENES IN THE THYMUS - LOCALIZATION OF TRANSCRIPTS INSITU AND COMPARISON OF MATURE AND IMMATURE SUBSETS

被引:16
作者
HELD, W
MUELLER, C
MACDONALD, HR
机构
[1] LUDWIG INST CANC RES, LAUSANNE BRANCH, CH-1066 EPALINGES, SWITZERLAND
[2] UNIV BERN, DEPT PATHOL, CH-3000 BERN, SWITZERLAND
关键词
D O I
10.1002/eji.1830200936
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have analyzed the expression of T cell receptor (TcR) genes in the thymus using in situ RNA hybridizations with probes to the constant regions of the TcR α, β, γ and δ chains. Localization of transcripts revealed low TcR α mRNA levels in the thymus cortex and very low levels in the subcapsular region. In contrast, TcR β message was very abundant in the cortex. TcR γ or δ mRNA+ thymocytes showed a scattered, predominantly cortical localization. In contrast to γ, TcR δ transcripts were abundant in the subcapsular region. Control experiments with sorted TcR α/β or γ/δ cells revealed a detection efficiency of 75%–85% for the respective TcR mRNA and data on TcR gene expression in mature, CD3+ thymocytes were consistent with previous reports. The analysis of immature, CD3− thymocyte subsets, however, revealed a virtual absence of TcR α transcripts and an unexpectedly high proportion of cells (14%–29%) expressing the gene for the TcR δ chain. The data are discussed in view of current models of lineage relationships in the thymus. Copyright © 1990 Wiley‐VCH Verlag GmbH & Co. KGaA
引用
收藏
页码:2133 / 2136
页数:4
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共 28 条
  • [21] PRODUCTION AND CHARACTERIZATION OF A RAT MONOCLONAL-ANTIBODY AGAINST THE MURINE CD3 MOLECULAR-COMPLEX
    MIESCHER, GC
    SCHREYER, M
    MACDONALD, HR
    [J]. IMMUNOLOGY LETTERS, 1989, 23 (02) : 113 - 118
  • [22] MIESCHER GC, 1988, J IMMUNOL, V140, P1779
  • [23] A HIGH PROPORTION OF LYMPHOCYTES-T THAT INFILTRATE INCOMPATIBLE-H-2 HEART ALLOGRAFTS INVIVO EXPRESS GENES ENCODING CYTO-TOXIC CELL-SPECIFIC SERINE PROTEASES, BUT DO NOT EXPRESS THE MEL-14-DEFINED LYMPH-NODE HOMING RECEPTOR
    MUELLER, C
    GERSHENFELD, HK
    LOBE, CG
    OKADA, CY
    BLEACKLEY, RC
    WEISSMAN, IL
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (03) : 1124 - 1136
  • [24] DIFFERENTIAL EXPRESSION OF 2 DISTINCT T-CELL RECEPTORS DURING THYMOCYTE DEVELOPMENT
    PARDOLL, DM
    FOWLKES, BJ
    BLUESTONE, JA
    KRUISBEEK, A
    MALOY, WL
    COLIGAN, JE
    SCHWARTZ, RH
    [J]. NATURE, 1987, 326 (6108) : 79 - 81
  • [25] EARLY GENETIC EVENTS IN T-CELL DEVELOPMENT ANALYZED BY INSITU HYBRIDIZATION
    PARDOLL, DM
    FOWLKES, BJ
    LECHLER, RI
    GERMAIN, RN
    SCHWARTZ, RH
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1987, 165 (06) : 1624 - 1638
  • [26] A MURINE EARLY THYMOCYTE DEVELOPMENTAL SEQUENCE IS MARKED BY TRANSIENT EXPRESSION OF THE INTERLEUKIN-2 RECEPTOR
    PEARSE, M
    WU, L
    EGERTON, M
    WILSON, A
    SHORTMAN, K
    SCOLLAY, R
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (05) : 1614 - 1618
  • [27] THE DIFFERENTIATION AND FUNCTION OF HUMAN LYMPHOCYTES-T
    REINHERZ, EL
    SCHLOSSMAN, SF
    [J]. CELL, 1980, 19 (04) : 821 - 827
  • [28] COMPLETE PRIMARY STRUCTURE OF A HETERODIMERIC T-CELL RECEPTOR DEDUCED FROM CDNA SEQUENCES
    SAITO, H
    KRANZ, DM
    TAKAGAKI, Y
    HAYDAY, AC
    EISEN, HN
    TONEGAWA, S
    [J]. NATURE, 1984, 309 (5971) : 757 - 762