RETINOBLASTOMA PROTEIN MONOCLONAL-ANTIBODIES WITH NOVEL CHARACTERISTICS

被引：24

作者：

WEN, SF

NODELMAN, M

NAREDHOOD, K

DUNCAN, J

GERADTS, J

SHEPARD, HM

机构：

[1] CANJI INC,DEPT ASSAY DEV,SAN DIEGO,CA 92121

[2] SCRIPPS LAB,SAN DIEGO,CA 92126

[3] UNIV N CAROLINA,SCH MED,DEPT PATHOL,CHAPEL HILL,NC 27599

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 169卷 / 02期

关键词：

RETINOBLASTOMA GENE PRODUCT; TUMOR SUPPRESSOR GENE; (DIAGNOSIS); (PROGNOSIS);

D O I：

10.1016/0022-1759(94)90267-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a family of monoclonal antibodies directed against the retinoblastoma gene product (p110(RB)). One of these monoclonal antibodies, 3C8, binds p110(RB) near the C-terminal end of the protein (aa886-aa905). It was characterized by immunoblotting, ELISA, fluorescence-activated flow cytometry and immunohistostaining. It was shown to be useful for the detection of p110(RB) in formalin-fixed and paraffin-embedded tissue sections. Because 3C8 binds outside of regions shown to be involved in p110(RB) interactions with other cellular proteins, it may be an especially useful reagent for the reliable detection of p110(RB) in tumor cells, and for the isolation by affinity chromatography of p110(RB) complexes with other cellular proteins.

引用

页码：231 / 240

页数：10

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