ENDOTHELINS INHIBIT LUTEINIZATION OF CULTURED PORCINE GRANULOSA-CELLS

被引:76
作者
IWAI, M
HASEGAWA, M
TAII, S
SAGAWA, N
NAKAO, K
IMURA, H
NAKANISHI, S
MORI, T
机构
[1] KYOTO UNIV,DEPT MED,DIV 2,SAKYO KU,KYOTO 606,JAPAN
[2] KYOTO UNIV,FAC MED,INST IMMUNOL,SAKYO KU,KYOTO 606,JAPAN
关键词
D O I
10.1210/endo-129-4-1909
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Endothelin (ET), a novel vasoconstrictor peptide containing three isopeptides [ET-1, ET-2, and ET-3 (ETs)], has various biological effects including vasoconstriction, mitogenesis, and steroidogenesis. We examined the ET-1-like immunoreactivity level in porcine follicular fluid and culture medium of porcine granulosa cells by RIA. The ET level in the follicular fluid was 9.4-14.2 pg/ml. These levels were within 0.42 to 0.62-fold of the porcine plasma level (22.7 +/- 3.1 pg/ml) (mean +/- SE). ET was detected in the culture medium of granulosa cells with and without LH treatment at the concentration of 56 +/- 9.3 and 4.9 +/- 1.2 pg/10(6) cells.h, respectively. We also examined whether ETs affect the luteinization of granulosa cells. ETs inhibited the LH-stimulated progesterone and cAMP accumulation in cultured porcine granulosa cells in a dose-dependent manner with an EC50 of 5 X 10(-11) M. ET-1, ET-2, and ET-3 (5 X 10(-8) M) inhibited progesterone accumulation by 62.3 +/- 1.8, 59.8 +/- 4.0, and 63.3 +/- 5.7% in 6-day cultures, respectively, and significant inhibition was observed within 24 h of culture. ET-1, ET-2, and ET-3 (5 X 10(-8) M) inhibited the LH-stimulated cAMP accumulation in granulosa cells by 54.8 +/- 2.3, 55.4 +/- 7.1, and 55.5 +/- 6.2%, respectively, whereas they did not affect basal cAMP levels. As well as progesterone accumulation, ETs partially inhibited LH-stimulated morphological transformation of granulosa cells. In this study, we demonstrated that ET exists in follicular fluid and in the culture medium of granulosa cells, and that ET inhibited LH-induced progesterone accumulation, morphological transformation, and cAMP accumulation in cultured porcine granulosa cells. These findings suggest that ET acts as a modulator of steroid metabolism in preovulatory follicles.
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页码:1909 / 1914
页数:6
相关论文
共 24 条
[1]   CLONING AND EXPRESSION OF A CDNA-ENCODING AN ENDOTHELIN RECEPTOR [J].
ARAI, H ;
HORI, S ;
ARAMORI, I ;
OHKUBO, H ;
NAKANISHI, S .
NATURE, 1990, 348 (6303) :730-732
[2]  
CANNING CP, 1983, FACTORS REGULATING O, P125
[3]   MORPHOLOGICAL-STUDIES OF THE MICROCIRCULATORY SYSTEM OF PERIOVULATORY OVINE FOLLICLES [J].
CAVENDER, JL ;
MURDOCH, WJ .
BIOLOGY OF REPRODUCTION, 1988, 39 (04) :989-997
[4]   ENDOTHELIN BINDING TO CULTURED CALF ADRENAL ZONA GLOMERULOSA CELLS AND STIMULATION OF ALDOSTERONE SECRETION [J].
COZZA, EN ;
GOMEZSANCHEZ, CE ;
FOECKING, MF ;
CHIOU, S .
JOURNAL OF CLINICAL INVESTIGATION, 1989, 84 (03) :1032-1035
[5]   ENDOTHELIN STIMULATES STEROID-SECRETION BY FROG ADRENAL-GLAND INVITRO - EVIDENCE FOR THE INVOLVEMENT OF PROSTAGLANDINS AND EXTRACELLULAR CALCIUM IN THE MECHANISM OF ACTION OF ENDOTHELIN [J].
DELARUE, C ;
DELTON, I ;
FIORINI, F ;
HOMODELARCHE, F ;
FASOLO, A ;
BRAQUET, P ;
VAUDRY, H .
ENDOCRINOLOGY, 1990, 127 (04) :2001-2008
[6]   SPECIFIC RECEPTORS FOR ENDOTHELIN-3 IN CULTURED BOVINE ENDOTHELIAL-CELLS AND ITS CELLULAR MECHANISM OF ACTION [J].
EMORI, T ;
HIRATA, Y ;
MARUMO, F .
FEBS LETTERS, 1990, 263 (02) :261-264
[7]   EFFECT OF MOUSE EPIDERMAL GROWTH-FACTOR ON DNA AND PROTEIN-SYNTHESIS, PROGESTERONE AND INHIBIN PRODUCTION BY BOVINE GRANULOSA-CELLS IN CULTURE [J].
FRANCHIMONT, P ;
HAZEEHAGELSTEIN, MT ;
CHARLETRENARD, C ;
JASPAR, JM .
ACTA ENDOCRINOLOGICA, 1986, 111 (01) :122-127
[8]   INTERLEUKIN-1 INHIBITS LUTEINIZATION OF PORCINE GRANULOSA-CELLS IN CULTURE [J].
FUKUOKA, M ;
MORI, T ;
TAII, S ;
YASUDA, K .
ENDOCRINOLOGY, 1988, 122 (01) :367-369
[9]   INHIBITORY EFFECTS OF INTERLEUKIN-1 ON LUTEINIZING HORMONE-STIMULATED ADENOSINE-3',5'-MONOPHOSPHATE ACCUMULATION BY CULTURED PORCINE GRANULOSA-CELLS [J].
FUKUOKA, M ;
TAII, S ;
YASUDA, K ;
TAKAKURA, K ;
MORI, T .
ENDOCRINOLOGY, 1989, 125 (01) :136-143
[10]   CLONING AND SEQUENCE-ANALYSIS OF CDNA-ENCODING THE PRECURSOR OF A HUMAN ENDOTHELIUM-DERIVED VASOCONSTRICTOR PEPTIDE, ENDOTHELIN - IDENTITY OF HUMAN AND PORCINE ENDOTHELIN [J].
ITOH, Y ;
YANAGISAWA, M ;
OHKUBO, S ;
KIMURA, C ;
KOSAKA, T ;
INOUE, A ;
ISHIDA, N ;
MITSUI, Y ;
ONDA, H ;
FUJINO, M ;
MASAKI, T .
FEBS LETTERS, 1988, 231 (02) :440-444