SEPARATION OF ALKALOIDS BY PH-ZONE-REFINING COUNTERCURRENT CHROMATOGRAPHY

被引：40

作者：

MA, Y ^{[1
]}

ITO, Y ^{[1
]}

SOKOLOSKY, E ^{[1
]}

FALES, HM ^{[1
]}

机构：

[1] NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 685卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)00667-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

pH-Zone-refining counter-current chromatography was applied to the separation of alkaloids from a crude extract of Crinum moorei using a multilayer coil planet centrifuge. After methyl tert.-butyl ether and water were equilibrated, triethylamine (5-10 mM) was added to the organic phase and hydrochloric acid (5-10 mM) to the aqueous phase. The separation was performed by eluting the column with either the organic phase (displacement mode) or the aqueous phase (reverse-displacement mode) while the other phase was used as the stationary phase. From 3 g of the extract, crinine, powelline and crinamidine were separated in 2.5-7 h with minimum overlapping.

引用

页码：259 / 262

页数：4

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