SEPARATION OF ALKALOIDS BY PH-ZONE-REFINING COUNTERCURRENT CHROMATOGRAPHY

被引:40
作者
MA, Y [1 ]
ITO, Y [1 ]
SOKOLOSKY, E [1 ]
FALES, HM [1 ]
机构
[1] NHLBI,BIOPHYS CHEM LAB,BETHESDA,MD 20892
关键词
D O I
10.1016/0021-9673(94)00667-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
pH-Zone-refining counter-current chromatography was applied to the separation of alkaloids from a crude extract of Crinum moorei using a multilayer coil planet centrifuge. After methyl tert.-butyl ether and water were equilibrated, triethylamine (5-10 mM) was added to the organic phase and hydrochloric acid (5-10 mM) to the aqueous phase. The separation was performed by eluting the column with either the organic phase (displacement mode) or the aqueous phase (reverse-displacement mode) while the other phase was used as the stationary phase. From 3 g of the extract, crinine, powelline and crinamidine were separated in 2.5-7 h with minimum overlapping.
引用
收藏
页码:259 / 262
页数:4
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