CDNA SEQUENCE OF 2 DISTINCT PITUITARY PROTEINS HOMOLOGOUS TO KEX2 AND FURIN GENE-PRODUCTS - TISSUE-SPECIFIC MESSENGER-RNAS ENCODING CANDIDATES FOR PRO-HORMONE PROCESSING PROTEINASES

被引：493

作者：

SEIDAH, NG ^{[1
]}

GASPAR, L ^{[1
]}

MION, P ^{[1
]}

MARCINKIEWICZ, M ^{[1
]}

MBIKAY, M ^{[1
]}

CHRETIEN, M ^{[1
]}

机构：

[1] CLIN RES INST MONTREAL, MOLEC NEUROENDOCRINOL LAB, MONTREAL H2W 1R7, QUEBEC, CANADA

来源：

DNA AND CELL BIOLOGY | 1990年 / 9卷 / 06期

关键词：

D O I：

10.1089/dna.1990.9.415

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Based on the concept of sequence conservation around the active sites of serine proteinases, polymerase chain reaction applied to mRNA amplification allowed us to obtain a 260-bp probe which was used to screen a mouse pituitary cDNA library. The primers used derived from the cDNA sequence of active sites Ser* and Asn* of human furin. Two cDNA sequences were obtained from a number of positive clones. These code for two similar but distinct structures (mPCl and mPC2), each being homologous to yeast Kex2 and human furin. In situ hybridization (mPCl) and Northern blots (mPCl = 3.0 kb and mPC2 = 2.8 and 4.8 kb) demonstrated tissue and cellular specificity of expression, only within endocrine and neuroendocrine cells. These data suggest that mPCl and mPC2 represent prime candidates for tissue-specific pro-hormone converting proteinases. © 1990, Mary Ann Liebert, Inc. All rights reserved.

引用

页码：415 / 424

页数：10