A NEW BACTERIAL CELLULOSE SUBSTRATE FOR MAMMALIAN-CELL CULTURE - A NEW BACTERIAL CELLULOSE SUBSTRATE

被引：81

作者：

WATANABE, K ^{[1
]}

ETO, Y ^{[1
]}

TAKANO, S ^{[1
]}

NAKAMORI, S ^{[1
]}

SHIBAI, H ^{[1
]}

YAMANAKA, S ^{[1
]}

机构：

[1] AJINOMOTO CO INC,CENT RES LABS,KAWASAKI KU,KAWASAKI,KANAGAWA 210,JAPAN

来源：

CYTOTECHNOLOGY | 1993年 / 13卷 / 02期

关键词：

ACETOBACTER; CELL; CELLULOSE; CULTURE; MAMMALIAN;

D O I：

10.1007/BF00749937

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new substrate for mammalian cell culture was developed using a cellulose membrane produced by Acetobacter aceti. Modification of the ionic charge of the membrane and adsorption of collagen to it promoted cellular adhesion to the membrane surface. The growth of eight kinds of cells on the membrane, was comparable to that achieved in plastic Petri dishes. The membrane was tested for use in the production of recombinant Erythroid Differentiation Factor (EDF)/activin A using genetically engineered Chinese hamster ovary cells. Both the viability of the cells and production of EDF/activin A were maintained for about 1 month, while cultures on plastic dishes lasted only 12 days. It was considered that the mechanism of improved cell viability was related to the ultrastructure of the cellulose membrane.

引用

页码：107 / 114

页数：8

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