ASSIGNMENT OF THE GENE FOR URIDINE-DIPHOSPHATE GALACTOSE-4-EPIMERASE TO HUMAN CHROMOSOME-1 BY HUMAN-MOUSE SOMATIC-CELL HYBRIDIZATION

被引：4

作者：

LIN, MS ^{[1
]}

OIZUMI, J ^{[1
]}

NG, WG ^{[1
]}

ALFI, OS ^{[1
]}

DONNELL, GN ^{[1
]}

机构：

[1] UNIV SO CALIF,DEPT PEDIAT,LOS ANGELES,CA 90027

来源：

SOMATIC CELL GENETICS | 1979年 / 5卷 / 03期

关键词：

D O I：

10.1007/BF01538848

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hybrid cell clones between mouse cells deficient in hypoxanthine phosphoribosyltransferase and human diploid cells were analyzed for the expression of human uridine diphosphate galactose-4-epimerase (UDPGal-4-epimerase, EC 5.1.3.2) and for the segregation of human chromosomes. Ten of the 18 independent hybrid clones analyzed were found to express human UDPGal-4-epimerase. Karyotype analysis of these hybrids showed concordant segregation of human UDPGal-4-epimerase with chromosome 1. Human UDPGal-4-epimerase is expressed as a dimeric structure demonstrated by the formation of an intermediate heterodimer migrating between the human and mouse forms in the cell hybrids. The gene specifying this enzyme was also observed to be syntenic with enolase-1. These results indicate that the structural gene for human UDPGal-4-epimerase is located on chromosome 1. © 1979 Plenum Publishing Corporation.

引用

页码：363 / 371

页数：9

共 24 条

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