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DEREPRESSION OF A PROLINE TRANSPORT SYSTEM IN SACCHAROMYCES CHEVALIERI BY NITROGEN STARVATION
被引:59
作者:
SCHWENCKE, J
MAGANASC.N
机构:
[1] Instituto de Quimica, Departamento de Ciencias, Universidad de Chile, Valparaiso
关键词:
D O I:
10.1016/0005-2736(69)90113-8
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
1. 1. Saccharomyces chevalieri cells grown in complex medium or in synthetic medium containing (NH4)2SO4 as the sole nitrogen source show a reduced capacity to accumulate [14C]proline. 2. 2. The uptake of proline is greatly enhanced by incubating the cells without further growth in a nitrogen-free medium, with galactose as the sole energy source. The enhancement in uptake capacity by nitrogen starvation is not affected by anaerobic or fully aerobic conditions. Also, the increase in proline pool is without effect. 3. 3. Cycloheximide, blasticidine-S and sparsomycin inhibit the derepression. Also, the amino acid analogs p-fluorophenylalanine and 5-methyltryptophan prevent the increase of proline uptake. Benzimidazole, proflavine and ethidium bromide are effective inhibitors of the derepression. 4. 4. Other amino acids do not show such an increase in accumulation capacity after nitrogen starvation. For some amino acids which show a moderate transport enhancement, such an increase is not abolished by cycloheximide. 5. 5. Results are discussed in terms of the idea that the biosynthesis of a protein necessary for the transport of proline is repressed during growth of Saccharomyces chevalieri and that derepression occurs when the cells are subjected to nitrogen starvation. © 1969.
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页码:302 / +
页数:1
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