SECRETION OF MULLERIAN INHIBITING SUBSTANCE BY CULTURED ISOLATED SERTOLI CELLS OF THE NEONATAL CALF

被引：31

作者：

PRICE, JM

机构：

[1] Department of Anatomy, University of Massachusetts Medical School, Worcester, Massachusetts 01605, 55 Lake Avenue, N.

来源：

AMERICAN JOURNAL OF ANATOMY | 1979年 / 156卷 / 01期

关键词：

D O I：

10.1002/aja.1001560116

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Sertoli cells have been isolated from the newborn calf testis using a combination of mechanical and enzymatic disruption. Testicular fragments, previously chopped into 1‐mm pieces, are digested in an enzyme mixture consisting of hyaluronidase, collagenase, trypsin and DNAse, followed by a second digestion in trypsin and DNAse. Isolation of the resulting cellular fractions by sedimentation with unit gravity produces an aliquot of Sertoli cells which is over 95% pure when examined by light and electron microscopy. Cultures of these cells grow rapidly and produce Mullerian Inhibiting Substance as evidenced by their ability to cause the involution of the Mullerian duct of the female fetal rat when co‐cultured in an organ‐culture assay system. Copyright © 1979 Wiley‐Liss, Inc.

引用

页码：147 / 157

页数：11

共 29 条

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