HYBRIDIZATION OF SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES TO PHI-CHI-174 DNA - EFFECT OF SINGLE BASE PAIR MISMATCH

被引：628

作者：

WALLACE, RB ^{[1
]}

SHAFFER, J ^{[1
]}

MURPHY, RF ^{[1
]}

BONNER, J ^{[1
]}

HIROSE, T ^{[1
]}

ITAKURA, K ^{[1
]}

机构：

[1] CALTECH,DIV BIOL,PASADENA,CA 91109

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 11期

关键词：

D O I：

10.1093/nar/6.11.3543

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligodeoxyribonucleotides complementary to the DNA of the wild type (wt) bacteriophage ΦX174 have been synthesized by the phosphotriester method. The ollgomers, 11, 14, and 17 bases long, are complementary to the region of the DNA which accounts for the am-3 point mutation. When hybridized to am-3 DNA, the oligonucleotides form duplexes with a single base pair mismatch. The thermal stability of the duplexes formed between wt and am-3 DNAs has been measured. The am-3 DNA: ollgomer duplexes dissociate at a temperature about 10°C lower than the corresponding wt DNA: ollgomer duplexes. This dramatic decrease in thermal stability due to a single mismatch makes it possible to eliminate the formation of the mismatched duplexes by the appropriate choice of hybridization temperature. These results are discussed with respect to the use of oligonucleotides as probes for the isolation of specific cloned DNA sequences. © 1979 Information Retrieval Limited.

引用

页码：3543 / 3557

页数：15

共 26 条

[11] UNITS OF TRANSCRIPTION AND TRANSLATION - SEQUENCE COMPONENTS OF HETEROGENEOUS NUCLEAR-RNA AND MESSENGER-RNA [J].