MUCOSAL DYSFUNCTION AND DAMAGE INDUCED BY PLATELET-ACTIVATING-FACTOR (PAF)

被引：27

作者：

GANBO, T

HISAMATSU, K

机构：

[1] Department of Otorhinolaryngology, Yamanashi College of Medicine, Yamanashi, 409-38, 1110 Shimogato, Tamahocho, Nakakoma-gun

来源：

ACTA OTO-LARYNGOLOGICA | 1990年 / 110卷 / 5-6期

关键词：

Ciliary inhibition; Cytotoxicity; Upper respiratory tract;

D O I：

10.3109/00016489009122570

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

The effect of PAF on human nasal mucosa was investigated in vitro. Normal paranasal sinus mucosa was obtained from the ethmoid sinuses by surgical procedure and incubated in the form of tissue culture. Ciliary movement was viewed under an inverted microscope and recorded on video tapes, and its activity was measured photoelectrically. Morphological alterations were examined by light and electron microscopy. PAF inhibited ciliary activity of human nasal mucosa, in a time and a dose dependent manner, at concentrations from 10-6M to 10-10M, while no significant change was observed at 10 M. Lyso-PAF exhibited minimal effect on the mucosa at a concentration of 10∼6M. Morphological alterations of the epithelial layer of the mucosa such as edema, cell exfoliation and desquamation were found to increase across time. Ultrastructural alterations were observed prior to inhibition of ciliary activity. These data indicate the cytotoxic effect of PAF on human paranasal sinus mucosa. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：427 / 436

页数：10

共 24 条

[11]

HAHN H L, 1985, American Review of Respiratory Disease, V131, pA27

[12]

HISAMATSU K, 1975, J OTOLARYNGOL JPN, V78, P16

[13]

HUMPHREY DM, 1984, LAB INVEST, V50, P16

[14] PROPERTIES OF PAF-ACETHER-INDUCED PLATELET-AGGREGATION AND SECRETION - STUDIES IN GEL-FILTERED HUMAN-PLATELETS [J].

KLOPROGGE, E ;

DEHAAS, GH ;

GORTER, G ;

AKKERMAN, JWN .

THROMBOSIS RESEARCH, 1983, 29 (06) :595-608

[15]

LEE TC, 1984, J BIOL CHEM, V259, P5526

[16]

LOTNER GZ, 1980, J IMMUNOL, V124, P676

[17] HUMAN-PLATELET STIMULATION BY ACETYL GLYCERYL ETHER PHOSPHORYLCHOLINE [J].

MCMANUS, LM ;

HANAHAN, DJ ;

PINCKARD, RN .

JOURNAL OF CLINICAL INVESTIGATION, 1981, 67 (03) :903-906

[18] EVIDENCE OF PAF-ACETHER METABOLIC PATHWAY ACTIVATION IN ANTIGEN CHALLENGE OF UPPER RESPIRATORY AIRWAYS [J].

MIADONNA, A ;

TEDESCHI, A ;

ARNOUX, B ;

SALA, A ;

ZANUSSI, C ;

BENVENISTE, J .

AMERICAN REVIEW OF RESPIRATORY DISEASE, 1989, 140 (01) :142-147

[19]

OKUDA M, 1983, ANN ALLERGY, V51, P51

[20]

SCHLEIMER RP, 1986, AM REV RESPIR DIS, V133, P614

← 1 2 3 →