1,25-DIHYDROXYVITAMIN-D3 RECEPTORS IN RAT-KIDNEY CYTOSOL

被引：87

作者：

CHANDLER, JS

PIKE, JW

HAUSSLER, MR

机构：

[1] Department of Biochemistry, University of Arizona, Tucson

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0006-291X(79)91933-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat kidney cytosol contains a 3.3 S high affinity binding component for 1,25-dihydroxyvitamin D3 as detected by DNA-cellulose chromatography and subsequent sucrose gradient analysis. The semipurified aporeceptor demonstrates specificity for 1,25-dihydroxyvitamin D3 and an apparent dissociation constant for this sterol-hormone of 3.4 × 10-10M at 25°C. The physicochemical properties of this binding component are in agreement with those observed for the chick intestinal 1,25-dihydroxyvitamin D3 receptor, suggesting that this component may function as a specific receptor for the hormone in the kidney. © 1979.

引用

页码：1057 / 1063

页数：7

共 20 条

[1]

Alberts B., 1971, METHOD ENZYMOL, V21, P198

[2]

BONJOUR JP, 1977, VITAMIN D BIOCH CHEM, P419

[3]

BRUMBAUGH PF, 1974, J BIOL CHEM, V249, P1258

[4]

CHANDLER JS, 1979, FED P, V37, P385