USE OF NITROCELLULOSE MEMBRANE TO ACTIVATE AND MEASURE INSECT PROPHENOL OXIDASE

被引：8

作者：

BREY, PT

STOLTZ, DB

COOK, DI

ASHIDA, M

机构：

[1] HOKKAIDO UNIV,INST LOW TEMP SCI,BIOCHEM LAB,SAPPORO,HOKKAIDO 060,JAPAN

[2] DALHOUSIE UNIV,DEPT MICROBIOL,HALIFAX B3H 4H7,NS,CANADA

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 194卷 / 02期

基金：

日本学术振兴会;

关键词：

D O I：

10.1016/0003-2697(91)90241-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the activation and measurement of insect prophenol oxidase using nitrocellulose membrane is presented. Using this method we were able to conveniently activate both crude and purified prophenol oxidase from insects belonging to three different orders. This rapid method allows for prophenol oxidase activation, in the absence of a prophenol oxidase-activating system, and in the presence of high ionic strength, protease inhibitors, or chelator. © 1991.

引用

页码：359 / 364

页数：6

共 30 条

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