SITE-SPECIFIC RECOMBINATION OF THE CIRCULAR 2-MU-M-LIKE PLASMID PKD1 REQUIRES INTEGRITY OF THE RECOMBINASE GENE-A AND OF THE PARTITIONING GENE-B AND GENE-C

In the circular plasmid pKD1, which stably replicates in Kluyveromyces lactis, the three open reading frames encode a site-specific recombinase (gene A) and two proteins involved in mitotic stability (genes B and C). A recombination analysis of plasmids in which gene B or C is inactivated reveals that unlike the 2mum plasmid of Saccharomyces cerevisiae, these genes are also required for the site specificity of plasmid recombination.