CHARACTERIZATION OF THE 18-KDA APPLE ALLERGEN BY 2-DIMENSIONAL IMMUNOBLOTTING AND MICROSEQUENCING

被引：90

作者：

VIETHS, S ^{[1
]}

SCHONING, B ^{[1
]}

PETERSEN, A ^{[1
]}

机构：

[1] FORSCHUNGSINST BORSTEL,DIV ALLERGOL,W-2061 BORSTEL,GERMANY

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 1994年 / 104卷 / 04期

关键词：

APPLE ALLERGENS; BIRCH POLLEN ALLERGENS; CROSS-REACTIVITY; IMMUNOBLOTTING; N-TERMINAL MICROSEQUENCING; 2-DIMENSIONAL ELECTROPHORESIS;

D O I：

10.1159/000236698

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

A low-temperature extract taken from Golden Delicious apples was separated by two-dimensional polyacrylamide gel electrophoresis. By means of two-dimensional immunoblotting with patients' serum containing IgE specific to Bet v I, a rabbit polyclonal antiserum raised against Bet v I, and two Bet v I specific monoclonal antibodies, epitopes cross-reactive to Bet v I were identified on an apple allergen with a molecular mass of 18 kDa and pI 5.5. Furthermore, certain antibody reactivities with 4 isoproteins of a molecular mass of 16 kDa and pIs ranging from 4.9 to 5.5 were observed, which may indicate the presence of Bet v I related epitopes on these proteins. Based on 26 amino acid residues, N-terminal sequencing of the 18-kDa apple allergen revealed 62% sequence identity between Bet v I from birch pollen and the apple allergen. Our results therefore support the view that both proteins express common as well as non-related IgE-reactive epitopes.

引用

页码：399 / 404

页数：6

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