EFFECT OF COOLING RATE, CRYOPROTECTANT AND HOLDING TIME AT DIFFERENT TRANSFER TEMPERATURES ON THE SURVIVAL OF CRYOPRESERVED CELL-SUSPENSION CULTURE (PUCCINELLIA-DISTANS (L) PARL)

被引:8
作者
HESZKY, LE [1 ]
JEKKEL, Z [1 ]
ALI, AH [1 ]
机构
[1] UNIV AGR SCI GODOLLO,DEPT GENET & PLANT BREEDING,H-2103 GODOLLO,HUNGARY
关键词
cooling rate; cryoprotectant; holding time; Puccinellia distans; suspension culture; transfer temperature;
D O I
10.1007/BF00047614
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Reflexed saltmarsh-grass suspension cultures produced by seed callus were frozen to the liquid nitrogen temperature. Cooling rates, cryoprotectants and holding times were taken as a function of transfer temperatures. The highest survival of cells (45%) was found at a freezing rate of 1°C min-1, without cryoprotectant treatments. The cryoprotectants (proline, dimethyl sulphoxide, glycerol), used at different concentrations and transfer temperatures, increased the survival rate. The maximum value was 78% at 12.5% (w/v) of proline with -30°C transfer temperature. Considerable improvement of viability (from 0% to 95%) among the 12.5 and 15.0% (v/v) dimethyl sulphoxide cryopreserved cells was achieved by holding them at - 20°C for 10-30 min before plunging into the liquid nitrogen. A 20 min holding time at 15.0% (v/v) glycerol level and - 30°C transfer temperature significantly enhanced the viability of the explants from 42% to 92%. Plants were successfully regenerated from cells cryopreserved with proline (w/v) and dimethyl sulfoxide (v/v) levels of 12.5 and 15.0%, respectively. © 1990 Kluwer Academic Publishers.
引用
收藏
页码:217 / 226
页数:10
相关论文
共 24 条
  • [1] ABE T, 1986, THEOR APPL GENET, V4, P3
  • [2] BAJAJ Y P S, 1977, Crop Improvement, V4, P48
  • [3] BAJAJ YPS, 1979, INDIAN J EXP BIOL, V17, P1405
  • [4] BAJAJ YPS, 1977, CROP IMPR, V4, P85
  • [5] PLANT-REGENERATION FROM CALLUS OF PUCCINELLIA-DISTANS (L) PARL
    BINH, DQ
    HESZKY, LE
    GYULAI, G
    KISS, E
    CSILLAG, A
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 1989, 18 (02) : 195 - 200
  • [6] CRYOPRESERVATION OF WHEAT SUSPENSION-CULTURE AND REGENERABLE CALLUS
    CHEN, THH
    KARTHA, KK
    GUSTA, LV
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 1985, 4 (02) : 101 - 109
  • [7] Chu C.C., 1978, P S PLANT TISS CULT, P45
  • [8] RESISTANCE TO FREEZING IN LIQUID-NITROGEN OF CARNATION (DIANTHUS-CARYOPHYLLUS L VAR EOLO) APICAL AND AXILLARY SHOOT TIPS EXCISED FROM DIFFERENT AGED INVITRO PLANTLETS
    DEREUDDRE, J
    FABRE, J
    BASSAGLIA, C
    [J]. PLANT CELL REPORTS, 1988, 7 (03) : 170 - 173
  • [9] EFFECTS OF CRYOPROTECTANTS IN COMBINATION ON THE SURVIVAL OF FROZEN SUGARCANE CELLS
    FINKLE, BJ
    ULRICH, JM
    [J]. PLANT PHYSIOLOGY, 1979, 63 (04) : 598 - 604
  • [10] CRYOPROTECTANT REMOVAL TEMPERATURE AS A FACTOR IN THE SURVIVAL OF FROZEN RICE AND SUGARCANE CELLS
    FINKLE, BJ
    ULRICH, JM
    [J]. CRYOBIOLOGY, 1982, 19 (03) : 329 - 335