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ANATOMY OF A DNA-REPLICATION FORK REVEALED BY RECONSTITUTION OF SV40 DNA-REPLICATION IN-VITRO

被引：516
作者：
WAGA, S
STILLMAN, B
机构：
[1] Cold Spring Harbor Laboratory, Cold Spring Harbor
来源：
NATURE | 1994年 / 369卷 / 6477期
关键词：
D O I：
10.1038/369207a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Complete enzymatic replication of DNA from the simian virus 40 origin has been reconstituted with T antigen and highly purified cellular proteins. DNA polymerase-alpha/primase functions primarily to synthesize RNA-DNA primers for initiation of DNA replication at the origin and for priming each Okazaki fragment. A polymerase switching mechanism requiring replication factor C and the proliferating cell nuclear antigen allows two molecules of DNA polymerase-delta to replicate both strands of the double helix conjointly.
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页码：207 / 212
页数：6
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