ANATOMY OF A DNA-REPLICATION FORK REVEALED BY RECONSTITUTION OF SV40 DNA-REPLICATION IN-VITRO

被引：516

作者：

WAGA, S

STILLMAN, B

机构：

[1] Cold Spring Harbor Laboratory, Cold Spring Harbor

来源：

NATURE | 1994年 / 369卷 / 6477期

关键词：

D O I：

10.1038/369207a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Complete enzymatic replication of DNA from the simian virus 40 origin has been reconstituted with T antigen and highly purified cellular proteins. DNA polymerase-alpha/primase functions primarily to synthesize RNA-DNA primers for initiation of DNA replication at the origin and for priming each Okazaki fragment. A polymerase switching mechanism requiring replication factor C and the proliferating cell nuclear antigen allows two molecules of DNA polymerase-delta to replicate both strands of the double helix conjointly.

引用

页码：207 / 212

页数：6

共 50 条

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