IONIC CURRENTS IN RETROGRADELY LABELED TRIGEMINOTHALAMIC NEURONS IN SLICES OF RAT MEDULLA

被引：10

作者：

CHEN, L

HUANG, LYM

机构：

[1] UNIV TEXAS,MED BRANCH,INST MARINE BIOMED,200 UNIV BLVD,SUITE 706A H-43,GALVESTON,TX 77550

[2] UNIV TEXAS,MED BRANCH,DEPT PHYSIOL & BIOPHYS,GALVESTON,TX 77550

来源：

NEUROSCIENCE LETTERS | 1990年 / 110卷 / 1-2期

关键词：

Calcium current; Fluorescent-labeled microsphere; Patch clamp; Potassium current; Sodium current; Thin brain slice; Trigeminothalamic neuron;

D O I：

10.1016/0304-3940(90)90788-B

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have recorded the ionic currents of identified trigeminothalamic neurons in medulla slices in vitro. Trigeminothalamic cells were first retrogradely labeled by injecting fluorescent latex microspheres in the thalamus of a 7- to 10-day-old rat. Two days later, thin slices (80-100 μm) were prepared from the lower medulla of the injected rat. Whole cell recordings were performed on the labeled cells located in the spinal trigeminal nucleus caudalis using the patch clamp technique. The voltage dependent inward sodium, inward calcium and outward potassium currents are qualitatively similar to those obtained from the enzymatically dissociated trigeminothalamic neurons. Successful application of this thin slice method opens the opportunity of studying synaptic circuitry in the trigeminothalamic system. © 1990.

引用

页码：66 / 71

页数：6