METHODICAL IMPROVEMENTS IN RYE ANTHER CULTURE

被引：5

作者：

FLEHINGHAUS, T ^{[1
]}

DEIMLING, S ^{[1
]}

GEIGER, HH ^{[1
]}

机构：

[1] UNIV HOHENHEIM,INST PLANT BREEDING POPULAT GENET & SEED SCI,POB 700 562-762,W-7000 STUTTGART 70,GERMANY

来源：

PLANT CELL REPORTS | 1991年 / 10卷 / 08期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The crucial problem in anther culture of rye (Secale cereale L.) is the very low regeneration capacity. Our study was conducted to overcome this restriction. The plant material used included a doubled haploid line (DH), two single crosses between DH lines, and a tetraploid Secale cereale L. population. The factors carbohydrate source, post-plating temperature treatment, and gelling agent were investigated. Substantial progress was achieved by substituting maltose for sucrose. Top rates of 49 % responding anthers and 20 % green plants were obtained from one of the single crosses after a post-plating cold treatment on gelrite solidified medium. We consider our results a methodical step forward in rye anther culture.

引用

页码：397 / 400

页数：4

共 26 条

[1]

BARLOY D, 1989, MAYDICA, V34, P303

[2] EFFECT OF MALTOSE ON THE RESPONSE OF POTATO ANTHERS IN CULTURE [J].