Biological Activity of Oligonucleotide-Poly(L-lysine) Conjugates: Mechanism of Cell Uptake

被引：168

作者：

Leonetti, Jean-Paul ^{[1
]}

Degols, Genevieve ^{[1
]}

Lebleu, Bernard ^{[1
]}

机构：

[1] Univ Montpellier 2, CNRS, UA 1191, Lab Biochim Prot, F-34060 Montpellier, France

来源：

BIOCONJUGATE CHEMISTRY | 1990年 / 1卷 / 02期

关键词：

D O I：

10.1021/bc00002a010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously shown that antisense oligomers linked to poly(L-lysine) (PLL) exhibit antiviral properties against vesicular stomatitis virus (VSV) at concentrations lower than 1 mu M. The conjugation to PLL provides an interesting alternative to natural or neutral oligomers to increase the biological effects of antisense oligomers. The internalization pathway of oligomer-PLL conjugates as compared to unconjugated oligomers has been studied in L929 cells. In parallel to their enhanced antiviral activity, PLL increases greatly the uptake of fluorescently tagged oligomers. This internalization follows a classical endocytic pathway and the oligomer has to be cleaved from PLL in the cell to exhibit an antiviral effect.

引用

页码：149 / 153

页数：5

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