A NOVEL PANCREATIC BETA-CELL ISOFORM OF CALCIUM CALMODULIN-DEPENDENT PROTEIN-KINASE-II (BETA(3) ISOFORM) CONTAINS A PROLINE-RICH TANDEM REPEAT IN THE ASSOCIATION DOMAIN

被引：48

作者：

URQUIDI, V

ASHCROFT, SJH

机构：

[1] Nuffield Department of Clinical Biochemistry, John Radcliffe Hospital, Oxford, OX3 9DU, Headington

来源：

FEBS LETTERS | 1995年 / 358卷 / 01期

基金：

英国惠康基金;

关键词：

CAM KINASE II; INSULIN SECRETION; PROTEIN PHOSPHORYLATION; BETA-CELL;

D O I：

10.1016/0014-5793(94)01381-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There is evidence for a role for calcium/calmodulin-dependent protein phosphorylation in regulation of insulin secretion but the molecular nature of the kinase(s) responsible is unknown. In this study, the screening of a neonatal rat islet cDNA library resulted in the isolation of a 2 kb clone that was 99% homologous to the beta' isoform of calcium/calmodulin-dependent protein kinase II. The predicted 589 amino acid sequence with a calculated mass of 64,976 Da contained a 24 amino acid deletion in addition to the 15 amino acid deletion that differentiates the beta' from the beta isoform, and included an 86 amino acid novel domain consisting of a tandem repeat of proline-rich residues. The expression of this new isoform of calcium/calmodulin-dependent protein kinase II (beta(3)) was confirmed in beta-cell lines and testis by DNA amplification of the sequence encoding the inserted domain by reverse transcriptase-polymerase chain reaction, followed by Southern analysis.

引用

页码：23 / 26

页数：4

共 34 条

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