ANALYSES OF MAB REACTIVE WITH PORCINE CD8

被引：44

作者：

SAALMULLER, A

AASTED, B

CANALS, A

DOMINGUEZ, J

GOLDMAN, T

LUNNEY, JK

MAURER, S

PESCOVITZ, MD

POSPISIL, R

SALMON, H

TREBICHAVSKY, I

VALPOTIC, I

VIZCAINO, JS

ZUCKERMANN, F

机构：

[1] ROYAL VET & AGR UNIV, DEPT VET VIROL & IMMUNOL, DK-1870 FREDERIKSBERG C, DENMARK

[2] INIA, DEPT SANIDAD ANIM, E-28012 MADRID, SPAIN

[3] USDA ARS, HELMINTH DIS LAB, BELTSVILLE, MD 20705 USA

[4] INDIANA UNIV, DEPT SURG & MICROBIOL IMMUNOL, INDIANAPOLIS, IN 46202 USA

[5] ACAD SCI CZECH REPUBL, INST MICROBIOL, DEPT IMMUNOL, CR-14220 PRAGUE, CZECH REPUBLIC

[6] INRA, IMMUNOL LAB, F-37380 MONNAIE, FRANCE

[7] UNIV ZAGREB, FAC VET, DEPT BIOL, ZAGREB 41000, CROATIA

[8] UNIV ILLINOIS, COLL VET MED, DEPT VET PATHOBIOL, URBANA, IL 61801 USA

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1994年 / 43卷 / 1-3期

关键词：

D O I：

10.1016/0165-2427(94)90144-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Among all mAb submitted to the first porcine CD workshop, based on FCM analyses six mAb could be identified to recognize the porcine CD8 analogue (workshop Nos. 004, 051, 052, 053, 108 and 109). In immunoprecipitation studies three mAb (Nos. 004, 108 and 109) recognized an antigen with an apparent molecular mass of about 35 kDa under reducing conditions and about 70 kDa under non-reducing conditions. The molecular masses of the antigens recognized by the three other mAb (Nos. 051, 052 and 053) are still unknown. Epitope analyses performed by blocking experiments led to the determination of two CD8 epitopes: CD8a and CD8b. CD8a is recognized by mAb Nos. 004, 051 and 052, and CD8b by Nos. 053, 108 and 109.

引用

页码：249 / 254

页数：6