SIMULTANEOUS DETERMINATION OF CATECHOLAMINES, THEIR BASIC METABOLITES AND SEROTONIN IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING A MIXED-MODE COLUMN AND AN 8-CHANNEL ELECTROCHEMICAL DETECTOR

被引:46
作者
MASHIGE, F
MATSUSHIMA, Y
MIYATA, C
YAMADA, R
KANAZAWA, H
SAKUMA, I
TAKAI, N
SHINOZUKA, N
OHKUBO, A
NAKAHARA, K
机构
[1] KYORITSU COLL PHARMACEUT SCI,MINATO KU,TOKYO 105,JAPAN
[2] TOKYO DENKI UNIV,FAC SCI & ENGN,HATOYAMA,SAITAMA 35003,JAPAN
[3] TOKYO MED COLL,SHINJUKU KU,TOKYO 160,JAPAN
[4] UNIV TOKYO,INST IND SCI,MINATO KU,TOKYO 106,JAPAN
关键词
D O I
10.1002/bmc.1130090506
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A high-performance liquid chromatographic method for simultaneous determination of free catecholamines, their basic metabolites and serotonin in human urine was developed. The compounds were separated on a precolumn of cation exchange resin and a mixed-mode (C-18/cation-exchange) column and determined by an eight-channel electrochemical detector. By this method, free norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NMN), metanephrine (MN), 3-methoxy-tyramine (3-MT) and serotonin (5-HT) were determined in 40 min with inter-assay precision of 1.9, 3.2, 1.2, 1.6, 1.6, 5.5 and 3.2%, respectively. Detection limits were between 0.05 (E) and 0.3 (3-MT) pmol. The mean values of analytical recovery for NE, E, DA, NMN, MN, 3-MT, 5-HT were 99, 104, 104, 99, 102, 109 and 99%, respectively.
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页码:221 / 225
页数:5
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