DIFFERENTIAL-EFFECTS OF TEMPERATURE AND EXOGENOUS FATTY-ACIDS ON MITOGEN-INDUCED PROLIFERATION IN CHANNEL CATFISH LYMPHOCYTE-T AND LYMPHOCYTE-B

被引:19
作者
BLY, JE [1 ]
BUTTKE, TM [1 ]
CLEM, LW [1 ]
机构
[1] E CAROLINA UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL,GREENVILLE,NC 27858
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY | 1990年 / 95卷 / 03期
关键词
D O I
10.1016/0300-9629(90)90242-K
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. 1. Exogenously supplied, BSA complexed, saturated and unsaturated fatty acids were compared for their effects on mitogen-induced DNA synthesis in channel catfish T and B lymphocytes. 2. 2. At "permissive" in vitro temperatures (27°C), high concentrations (≥ 240 μM) of all the fatty acids used were inhibitory. However, at lower concentrations (80-160 μM), differences were noted in the ability of some fatty acids to modulate mitogen responses. While palmitic acid (16:0) and linoleic acid (18:2) had little effect on LPS-induced B cell- or Con A-indueed T cell proliferation, stearic acid (18:0) suppressed while oleic acid (18:1) enhanced T cell responses only. 3. 3. Adding equimolar amounts of 18:0 and 18:1 obviated the effects of singularly added fatty acids on T cell mitogenesis. 4. 4. 18:1 was used to successfully "rescue" ~ 60% of the Con A-induced T cell proliferation normally inhibited at "nonpermissive" in vitro temperatures (17°C). 5. 5. While B cells readily appear to desaturate 18:0 and synthesize unsaturated fatty acids, T cells accumulate comparatively large amounts of 18:0 in membrane associated phospholipids. 6. 6. It is proposed that 18:1 enhances T cell responses at permissive high temperatures and rescues suppressed T cell responses at nonpermissive low temperatures by increasing membrane fluidity. © 1990.
引用
收藏
页码:417 / 424
页数:8
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