THE EFFECT OF METHYL-LIDOCAINE ON THE BIOSYNTHESIS OF PHOSPHOLIPIDS DENOVO IN THE ISOLATED HAMSTER HEART

被引：17

作者：

TARDI, PG ^{[1
]}

MAN, RYK ^{[1
]}

CHOY, PC ^{[1
]}

机构：

[1] UNIV MANITOBA,FAC MED,DEPT BIOCHEM & MOLEC BIOL,WINNIPEG R3E 0W3,MANITOBA,CANADA

来源：

BIOCHEMICAL JOURNAL | 1992年 / 285卷

关键词：

D O I：

10.1042/bj2850161

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methyl-lidocaine is an amphiphilic agent which has been used as an experimental anti-arrhythmic drug. When hamster hearts were perfused with labelled glycerol, the presence of methyl-lidocaine in the perfusate was found to enhance the labelling in phosphatidylserine, phosphatidylinositol, diacylglycerol and triacylglycerol. However, the labelling of phosphatidylcholine and phosphatidylethanolamine was not significantly changed by methyl-lidocaine treatment. Assays in vitro for the enzymes involved in the synthesis of neutral lipids and acidic phospholipids revealed that phosphatidate phosphatase and CTP:phosphatidate cytidylyltransferase activities were stimulated by methyl-lidocaine. The intracellular pool sizes of diacylglycerol and CDP-diacylglycerol were also elevated. We postulate that the enhanced syntheses of the neutral lipids and acidic phospholipids in the methyl-lidocaine-perfused heart were mediated via the direct activation of the key enzymes in the biosynthesis of these lipids de novo.

引用

页码：161 / 166

页数：6

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