REPLACEMENT OF THE PROMOTER OF THE YEAST PLASMA-MEMBRANE ATPASE GENE BY A GALACTOSE-DEPENDENT PROMOTER AND ITS PHYSIOLOGICAL CONSEQUENCES

被引：117

作者：

CID, A ^{[1
]}

PERONA, R ^{[1
]}

SERRANO, R ^{[1
]}

机构：

[1] UNIV AUTONOMA, FAC MED,CSIC,INST INVEST BIOMED, ARZOBISPO MORCILLO 4, E-28029 MADRID, SPAIN

来源：

CURRENT GENETICS | 1987年 / 12卷 / 02期

关键词：

D O I：

10.1007/BF00434664

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In order to probe the physiological role of the yeast [Saccharomyces cerevisiae] plasma membrane ATPase we have replaced the constitutive promoter of its gene by a galactose-dependent promoter. The resulting cells stop growing on glucose medium when the preformed ATPase is diluted to 20% of normal. There is a correlation between ATPase activity and both proton efflux from the cells and amino acid transport. A large proportion of growth-arrested cells appear enlarged and with several buds containing nuclei.

引用

页码：105 / 110

页数：6

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