MOBILITY OF SINGLE-STRANDED-DNA AS A FUNCTION OF CROSS-LINKER CONCENTRATION IN POLYACRYLAMIDE CAPILLARY GEL-ELECTROPHORESIS

被引：26

作者：

FIGEYS, D ^{[1
]}

DOVICHI, NJ ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON T6G 2G2,ALBERTA,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 645卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1016/0021-9673(93)83391-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mobility, theoretical plate count, and resolution are reported for single-stranded DNA in polyacrylamide containing 0, 0.5, 2.5, and 5% cross-linker and 6% total acrylamide. An electric field of 300 V/cm was used with 50 mum I.D. capillaries. Mobility decreases with increasing cross-linker concentration. The transition from normal mobility to the limiting mobility of biased reptation with stretching occurs for longer fragments as the %C is decreased. Theoretical plate counts do not vary significantly between the different polyacrylamide compositions. Resolution is higher for 0%C polyacrylamide, due to the latter onset of biased reptation with stretching.

引用

页码：311 / 317

页数：7

共 26 条

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