ANALYSIS OF BILE-ACIDS AND THEIR CONJUGATES USING HIGH-PH ANION-EXCHANGE CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION

被引：13

作者：

CHAPLIN, MF

机构：

[1] School of Applied Science, South Bank University, London, SE1 0AA

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 664卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00488-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bile acids and their conjugated forms may be separated by anion-exchange chromatography in alkaline media (0.9 M sodium acetate, 0.1 M sodium hydroxide, 15% v/v acetonitrile) on a CarboPac PA-100 column. The effluent was monitored at high sensitivity, with detection limits of less than 10 mu M, using a pulsed amperometric detector. Free bile acids and their glyco- and tauro-conjugated forms were separated and detected within 40 min under isocratic conditions.

引用

页码：431 / 434

页数：4

共 9 条

[1]

DRISCOLL TR, 1992, BRIT J IND MED, V49, P700

[2] LONG-TERM DOUBLE-BLIND-STUDY ON THE INFLUENCE OF DIETARY-FIBERS ON FECAL BILE-ACID EXCRETION IN JUVENILE ULCERATIVE-COLITIS [J].