THE THIOESTERASE OF THE ERYTHROMYCIN-PRODUCING POLYKETIDE SYNTHASE - MECHANISTIC STUDIES IN-VITRO TO INVESTIGATE ITS MODE OF ACTION AND SUBSTRATE-SPECIFICITY

被引：48

作者：

AGGARWAL, R

CAFFREY, P

LEADLAY, PF

SMITH, CJ

STAUNTON, J

机构：

[1] DEPT CHEM,CTR MOLEC RECOGNIT,CAMBRIDGE CB2 1EW,ENGLAND

[2] DEPT BIOCHEM,CTR MOLEC RECOGNIT,CAMBRIDGE CB2 1QW,ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS | 1995年 / 15期

关键词：

D O I：

10.1039/c39950001519

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In vitro studies of the mode of action of the thioesterase of the erythromycin-producing PKS have shown that it cleaves ester groups by forming acyl enzyme intermediates, and that it has a broad substrate specificity.

引用

页码：1519 / 1520

页数：2

共 7 条

[1] A MUTANT GENERATED BY EXPRESSION OF AN ENGINEERED DEBS1 PROTEIN FROM THE ERYTHROMYCIN-PRODUCING POLYKETIDE SYNTHASE (PKS) IN STREPTOMYCES-COELICOLOR PRODUCES THE TRIKETIDE AS A LACTONE, BUT THE MAJOR PRODUCT IS THE NOR-ANALOG DERIVED FROM ACETATE AS STARTER ACID
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[2] AN ACYL-CARRIER-PROTEIN-THIOESTERASE DOMAIN FROM THE 6-DEOXYERYTHRONOLIDE-B SYNTHASE OF SACCHAROPOLYSPORA-ERYTHRAEA - HIGH-LEVEL PRODUCTION, PURIFICATION AND CHARACTERIZATION IN ESCHERICHIA-COLI
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[5] MODULAR ORGANIZATION OF GENES REQUIRED FOR COMPLEX POLYKETIDE BIOSYNTHESIS
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[6] BIOSYNTHESIS OF THE ERYTHROMYCIN MACROLACTONE AND A RATIONAL APPROACH FOR PRODUCING HYBRID MACROLIDES
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[7] THE EXTRAORDINARY ENZYMES INVOLVED IN ERYTHROMYCIN BIOSYNTHESIS
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