REGULATED PRODUCTION OF RECOMBINANT ECHISTATIN BY YEAST

被引：6

作者：

CARTY, CE ^{[1
]}

HOFMANN, KJ ^{[1
]}

KELLER, PM ^{[1
]}

POLOKOFF, MA ^{[1
]}

LYNCH, RJ ^{[1
]}

KEECH, BJ ^{[1
]}

GOULD, RJ ^{[1
]}

MAIGETTER, RZ ^{[1
]}

SCHULTZ, LD ^{[1
]}

机构：

[1] MERCK SHARP & DOHME LTD,DEPT BIOL CHEM,W POINT,PA 19486

来源：

BIOTECHNOLOGY LETTERS | 1990年 / 12卷 / 12期

关键词：

D O I：

10.1007/BF01022583

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The alpha-mating factor pre-pro-leader sequence under the regulation of the GAL10 promoter was used to direct the secretion of echistation by recombinant yeasts. Optimization of the culture medium and host strain increased the productivity of shake flask cultures twenty-fold to 8 mg/L. In fermentors, the production of echistatin was greater than 40 mg/L.

引用

页码：879 / 884

页数：6