DETERMINATION OF CHOLINESTERASE IN BLOOD-SERUM WITH A BENZOATE-SENSITIVE MEMBRANE-ELECTRODE

被引：12

作者：

KATSU, T

KAYAMOTO, T

机构：

[1] Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 254卷 / 1-2期

关键词：

POTENTIOMETRY; BLOOD; CHOLINESTERASE ASSAY; ION-SELECTIVE ELECTRODES;

D O I：

10.1016/0003-2670(91)90014-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Benzoic acid, formed by the enzymatic hydrolysis of benzoylcholine with cholinesterase, is detected with a benzoate-sensitive membrane electrode made with tridodecylmethylammonium chloride as ion-exchanger and omicron-nitrophenyl octyl ether as plasticizer. The detection limits were 0.02 mM benzoate in phosphate buffer at pH 7.4 and 0.1 mM in the presence of blood serum. Cholinesterase in blood serum was determined successfully by using this electrode.

引用

页码：95 / 97

页数：3

共 7 条

[1] GENERAL ENZYME STUDIES WITH A SUBSTRATE-SELECTIVE ELECTRODE - CHARACTERIZATION OF CHOLINESTERASES [J].