SIGMA-LIKE TRANSCRIPTION FACTORS FROM MUSTARD (SINAPIS-ALBA L) ETIOPLAST ARE SIMILAR IN SIZE TO, BUT FUNCTIONALLY DISTINCT FROM, THEIR CHLOROPLAST COUNTERPARTS

Three proteins resembling bacterial sigma factors were previously isolated from mustard chloroplasts (K. Tiller, A. Eisermann and G. Link, Eur J Biochem 198: 93-99, 1991). These sigma-like factors (SLFs) confer DNA-binding and transcription specificity to a system consisting of Escherichia coli core RNA polymerase and cloned DNA regions that carry a chloroplast promoter. Sigma-like activity was now isolated also from etioplasts and could be assigned to three polypeptides of M(r) 67 000 (SLF67), 52000 (SLF52) and 29000 (SLF29), i.e. the same sizes as for the chloroplast SLFs. The purification scheme for the factors from either plastid type included an initial heparin-Sepharose and a final gel filtration step. For the etioplast factors, however, an additional phosphocellulose step was required to release these polypeptides from the RNA polymerase. The etioplast SLFs have similar, but not identical, salt requirements for DNA binding as compared to their chloroplast counterparts. Under conditions of maximum binding activity there is overall preference of etioplast SLFs for the psbA promoter over the trnQ and rps16 promoters.