HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF RUTAECARPINE IN RAT PLASMA - APPLICATION TO A PHARMACOKINETIC STUDY

被引：26

作者：

KO, HC

TSAI, TH

CHOU, CJ

HSU, SY

LI, SY

CHEN, CF

机构：

[1] NATL YANG MING MED COLL,INST PHARMACOL,TAIPEI 112,TAIWAN

[2] NATL RES INST CHINESE MED,TAIPEI,TAIWAN

[3] CHINESE CULTURE UNIV,INST APPL CHEM,TAIPEI,TAIWAN

[4] NATL YANG MING MED COLL,INST TRADIT MED,TAIPEI 112,TAIWAN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 655卷 / 01期

关键词：

D O I：

10.1016/S0378-4347(94)80128-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and sensitive high-performance liquid chromatographic method for determination and identification of rutaecarpine in rat plasma has been developed. Up to 0.1 ml of plasma containing rutaecarpine was deproteinized by acetonitrile, which contained an internal standard (paeonol). The supernatant was injected onto a reversed-phase column using a acetonitrile-water-orthophosphoric acid (85%) (60:40:0.1, v/v/v, pH 2.5-2.8) as the mobile phase and ultraviolet detection at 344 nm. It was applied to the pharmacokinetic study of rutaecarpine in rat after a 2 mg/kg intravenous administration. A biphasic process with a rapid distribution followed by a slower elimination phase was observed from the plasma concentration-time curve. Compartmental analysis yielded a two-compartment model.

引用

页码：27 / 31

页数：5