LEUKOCYTE DNA-SYNTHESIS IN GRAFTED LUMBRICIDS - APPROACH TO STUDY HISTOCOMPATIBILITY IN INVERTEBRATES

被引：17

作者：

ROCH, P ^{[1
]}

机构：

[1] UNIV BORDEAUX 1,ZOOL LAB A,F-33405 TALENCE,FRANCE

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 1979年 / 3卷 / 03期

关键词：

D O I：

10.1016/S0145-305X(79)80038-5

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

We describe a simple technique for quickly determining graft reactivity in the earthworm Eisenia fetida andrei by incubating leukocytes for 16 hours in vitro with 3H-thymidine. This in vitro technique does not kill the reacting worms and the anesthesia (chloroform and low temperature) caused neither muscular contractions nor loss of coelomocytes. Stimulation of DNA-synthesis induced by allo- or xeno-antigens was only detected in the subpopulation of non-adherent leukocytes (NAL). Maximum stimulation was measured 4 days after first-set grafting in allo- as well as in xeno-combinations and, in both cases, the magnitudes of NAL incorporations were comparable. © 1979 Pergamon Press Ltd. All rights reserved.

引用

页码：417 / 428

页数：12

共 24 条

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