AFFINITY CHROMATOGRAPHIC PURIFICATION OF BOVINE LUNG ENDOTHELIN RECEPTOR USING BIOTINYLATED ENDOTHELIN AND AVIDIN AGAROSE

被引：12

作者：

HAGIWARA, H

NAGASAWA, T

LODHI, KM

KOZUKA, M

ITO, T

HIROSE, S

机构：

[1] Department of Biological Sciences, Tokyo Institute of Technology, Meguroku, Tokyo, 152, Ookayama

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 597卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)80128-H

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Endothelin receptor was purified from bovine lung by affinity chromatography using biotinylated endothelin and avidin agarose. Endothelin was biotinylated with sulphosuccinimidyl 2-(biotinamido)ethyl-1,3-dithiopropionate, a reactive form of biotin with a cleavable spacer arm containing a disulphide bond designed for a simple elution by thiols. Starting from 3.5 kg of bovine lung, about 200-mu-g of pure receptor were obtained.

引用

页码：331 / 334

页数：4

共 17 条

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