REGULATION OF KERATINOCYTE GROWTH, DIFFERENTIATION, AND VITAMIN-D METABOLISM BY ANALOGS OF 1,25-DIHYDROXYVITAMIN-D

被引:48
作者
BIKLE, DD [1 ]
GEE, E [1 ]
PILLAI, S [1 ]
机构
[1] UNIV CALIF SAN FRANCISCO,DEPT MED,SAN FRANCISCO,CA 94143
关键词
CALCITRIOL; VITAMIN-D ANALOGS; KERATINOCYTES; CORNIFIED ENVELOPE; PROLIFERATION; VITAMIN-D RECEPTOR;
D O I
10.1111/1523-1747.ep12371681
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
1,25(OH)2D has numerous actions on many tissues. Analogs of 1,25(OH)2D are being sought that are selective, to further an understanding of the mechanisms of action of 1,25(OH)2D and to improve its therapeutic efficacy. Toward these ends we examined eight analogs of 1,25(OH)2D for their ability to regulate 25OHD metabolism by keratinocytes. Choosing the three most potent, we then examined their ability to inhibit keratinocyte proliferation, stimulate cornified envelope formation (a marker of differentiation), and bind to the 1,25(OH)2D receptor (VDR). 1,25(OH)2-24F2-D, 1,25(OH)2-DELTA16-D, and 1,25(OH)2-DELTA16,23yne-D proved the most potent in inhibiting 1,25(OH)2D production and stimulating 24,25(OH)2D production, being approximately 10-100 times more potent than 1,25(OH)2D itself. 1,25(OH)2-DELTA16-D had the highest affinity for the VDR (fourfold higher than that for that for 1,25(OH)2D itself) and had the greatest ability both to inhibit proliferation and to stimulate differentiation. 1,25(OH)2-DELTA16,23yne-D also had a higher affinity for the VDR but was of less or equal potency in stimulating cornified envelope formation and inhibiting proliferation. 1,25(OH)2-24F2D2, which was the most potent regulator of 25OHD metabolism, had a lower affinity for the VDR and was less potent than 1,25(OH)2D in inhibiting proliferation. Our results indicate that even in the same cell different analogs have different rank orders of potency for the various actions of 1,25(OH)2D.
引用
收藏
页码:713 / 718
页数:6
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