DETERMINATION OF THE CONCENTRATIONS OF 5-FLUOROURACIL AND ITS METABOLITES IN RABBIT PLASMA AND TISSUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：20

作者：

DELNOZAL, MJ ^{[1
]}

BERNAL, JL ^{[1
]}

PAMPLIEGA, A ^{[1
]}

MARINERO, P ^{[1
]}

POZUELO, M ^{[1
]}

机构：

[1] UNIV VALLADOLID,FAC MED,DEPT INTERNAL MED,E-47005 VALLADOLID,SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 656卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00126-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The concentrations of 5-fluorouracil, 5-fluoro-5,6-dihydrouracil, 5-fluorouridine and 5-fluoro-2'-deoxyuridine in plasma, liver, kidney, lung and heart of rabbits were determined by high-performance liquid chromatography (HPLC) after drug administration by two different routes. HPLC was carried out by using a Spherisorb 5 ODS 2 column and 0.05 M phosphate buffer as the mobile phase with UV detection at 200 nm. The pH of the mobile phase, organic modifier content and column temperature were found to have a profound influence on the results, hence it was necessary to optimize a procedure for each matrix. A comparison of the efficiency of intravenous and peritoneal administration revealed that the latter provides higher drug concentrations in the liver and minimal contents in plasma and all other tissues studied.

引用

页码：397 / 405

页数：9

共 18 条

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