SEQUENCE-DEPENDENT CONFORMATIONAL VARIATIONS IN THE B-DNA DOUBLE-HELIX OF POLY-D(AATT)CENTER-DOT-POLY-D(AATT)

被引:10
作者
CHANDRASEKARAN, R [1 ]
RADHA, A [1 ]
RATLIFF, RL [1 ]
机构
[1] LOS ALAMOS NATL LAB,DIV LIFE SCI,GENET GRP,LOS ALAMOS,NM 87545
基金
美国国家卫生研究院;
关键词
D O I
10.1080/07391102.1994.10508030
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
X-ray diffraction data from well oriented and polycrystalline fibers of the lithium salt of poly d(AATT).poly d(AATT? are isomorphous with those from B-DNA. The double-helix consists of conformationally identical antiparallel strands and the molecular symmetry is 2 5(2); the asymmetric unit is a tetranucleotide, AATT, and 5 tetranucleotides span two turns per strand. Two double helices pass through a monoclinic unit cell of dimensions a = 31.05, b = 22.62, c = 33.85 Angstrom (fiber axis) and gamma = 90 degrees. In each repeating motif, the four nucleotides have distinct conformations, TpA displays an axial P-O bond and there is shortening of minor groove in the central region.
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页码:741 / 766
页数:26
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