MACROPHAGE NO2- PRODUCTION AS A SENSITIVE AND RAPID ASSAY FOR THE QUANTITATION OF MURINE IFN-GAMMA

被引：82

作者：

MIGLIORINI, P ^{[1
]}

CORRADIN, G ^{[1
]}

CORRADIN, SB ^{[1
]}

机构：

[1] UNIV LAUSANNE,INST BIOCHEM,CH-1066 EPALINGES,SWITZERLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 139卷 / 01期

关键词：

INTERFERON-GAMMA; NITRITE PRODUCTION; BIOASSAY; RAW CELL;

D O I：

10.1016/0022-1759(91)90357-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Macrophage production of arginine-derived NO-2 provides a simple method for detection and quantitation of interferon-gamma (IFN-gamma) in murine cell culture fluids. When the macrophage cell line RAW 264 is cultured overnight with IFN-gamma in the presence of 10 ng/ml LPS, NO-2 release, as determined by a simple colorimetric assay, is proportional to the concentration of IFN-gamma and is inhibited by monoclonal antibody to IFN-gamma. A high degree of correlation was obtained when antigen stimulated T lymphocyte supernatants were tested for IFN-gamma by ELISA and the NO-2 production assay.

引用

页码：107 / 114

页数：8

共 22 条

[1] MONOCLONAL-ANTIBODY 2-SITE ELISA FOR HUMAN IFN-GAMMA - ADAPTATION FOR DETERMINATIONS IN HUMAN-SERUM OR PLASMA [J].

ANDERSSON, G ;

EKRE, HPT ;

ALM, G ;

PERLMANN, P .

JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 125 (1-2) :89-96

[2] USE OF MONOCLONAL-ANTIBODIES AS SENSITIVE AND SPECIFIC PROBES FOR BIOLOGICALLY-ACTIVE HUMAN GAMMA-INTERFERON [J].

CHANG, TW ;

MCKINNEY, S ;

LIU, V ;

KUNG, PC ;

VILCEK, J ;

LE, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (16) :5219-5222

[3]

DING A, 1990, J IMMUNOL, V145, P940

[4]

DING AH, 1988, J IMMUNOL, V141, P2407

[5]

DRAPIER JC, 1988, J IMMUNOL, V140, P2829

[6] ENZYME-LINKED BIO-IMMUNOASSAY FOR IFN-GAMMA BY HLA-DR INDUCTION [J].

GIBSON, UEM ;

KRAMER, SM .

JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 125 (1-2) :105-113

[7] ANALYSIS OF NITRATE, NITRITE, AND [N-15]-LABELED NITRATE IN BIOLOGICAL-FLUIDS [J].

GREEN, LC ;

WAGNER, DA ;

GLOGOWSKI, J ;

SKIPPER, PL ;

WISHNOK, JS ;

TANNENBAUM, SR .

ANALYTICAL BIOCHEMISTRY, 1982, 126 (01) :131-138

[8]

HAAKFRENDSCHO M, 1990, CLIN EXP IMMUNOL, V82, P404

[9]

JAMES SL, 1990, J IMMUNOL, V145, P2686

[10] ESTABLISHMENT OF MOUSE-CELL LINES WHICH CONSTITUTIVELY SECRETE LARGE QUANTITIES OF INTERLEUKIN-2, INTERLEUKIN-3, INTERLEUKIN-4 OR INTERLEUKIN-5, USING MODIFIED CDNA EXPRESSION VECTORS [J].

KARASUYAMA, H ;

MELCHERS, F .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (01) :97-104

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