MACROPHAGE NO2- PRODUCTION AS A SENSITIVE AND RAPID ASSAY FOR THE QUANTITATION OF MURINE IFN-GAMMA

被引:82
作者
MIGLIORINI, P [1 ]
CORRADIN, G [1 ]
CORRADIN, SB [1 ]
机构
[1] UNIV LAUSANNE,INST BIOCHEM,CH-1066 EPALINGES,SWITZERLAND
关键词
INTERFERON-GAMMA; NITRITE PRODUCTION; BIOASSAY; RAW CELL;
D O I
10.1016/0022-1759(91)90357-L
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Macrophage production of arginine-derived NO-2 provides a simple method for detection and quantitation of interferon-gamma (IFN-gamma) in murine cell culture fluids. When the macrophage cell line RAW 264 is cultured overnight with IFN-gamma in the presence of 10 ng/ml LPS, NO-2 release, as determined by a simple colorimetric assay, is proportional to the concentration of IFN-gamma and is inhibited by monoclonal antibody to IFN-gamma. A high degree of correlation was obtained when antigen stimulated T lymphocyte supernatants were tested for IFN-gamma by ELISA and the NO-2 production assay.
引用
收藏
页码:107 / 114
页数:8
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