MACROPHAGE NO2- PRODUCTION AS A SENSITIVE AND RAPID ASSAY FOR THE QUANTITATION OF MURINE IFN-GAMMA

被引：82

作者：

MIGLIORINI, P ^{[1
]}

CORRADIN, G ^{[1
]}

CORRADIN, SB ^{[1
]}

机构：

[1] UNIV LAUSANNE,INST BIOCHEM,CH-1066 EPALINGES,SWITZERLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 139卷 / 01期

关键词：

INTERFERON-GAMMA; NITRITE PRODUCTION; BIOASSAY; RAW CELL;

D O I：

10.1016/0022-1759(91)90357-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Macrophage production of arginine-derived NO-2 provides a simple method for detection and quantitation of interferon-gamma (IFN-gamma) in murine cell culture fluids. When the macrophage cell line RAW 264 is cultured overnight with IFN-gamma in the presence of 10 ng/ml LPS, NO-2 release, as determined by a simple colorimetric assay, is proportional to the concentration of IFN-gamma and is inhibited by monoclonal antibody to IFN-gamma. A high degree of correlation was obtained when antigen stimulated T lymphocyte supernatants were tested for IFN-gamma by ELISA and the NO-2 production assay.

引用

页码：107 / 114

页数：8

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