学术探索
学术期刊
新闻热点
数据分析
智能评审

SPONTANEOUS ASSEMBLY OF A SELF-COMPLEMENTARY OLIGOPEPTIDE TO FORM A STABLE MACROSCOPIC MEMBRANE

被引：1065
作者：
ZHANG, SG
HOLMES, T
LOCKSHIN, C
RICH, A
机构：
[1] MIT, DEPT BIOL 16-739, CAMBRIDGE, MA 02139 USA
[2] MIT, DEPT BRAIN & COGNIT SCI, CAMBRIDGE, MA 02139 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 08期
关键词：
BETA-SHEET; INSOLUBLE FILAMENTS; IONIC BONDS; ORIGIN OF LIFE; ZUOTIN;
D O I：
10.1073/pnas.90.8.3334
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A 16-residue peptide [(Ala-Glu-Ala-Glu-Ala-Lys-Ala-Lys)2] has a characteristic beta-sheet circular dichroism spectrum in water. Upon the addition of salt, the peptide spontaneously assembles to form a macroscopic membrane. The membrane does not dissolve in heat or in acidic or alkaline solutions, nor does it dissolve upon addition of guanidine hydrochloride, SDS/urea, or a variety of proteolytic enzymes. Scanning EM reveals a network of interwoven filaments almost-equal-to 10-20 nm in diameter. An important component of the stability is probably due to formation of complementary ionic bonds between glutamic and lysine side chains. This phenomenon may be a model for studying the insoluble peptides found in certain neurological disorders. It may also have implications for biomaterials and origin-of-life research.
引用
收藏
页码：3334 / 3338
页数：5
相关论文
共 24 条
[1]   SOLUTION STRUCTURES OF BETA PEPTIDE AND ITS CONSTITUENT FRAGMENTS - RELATION TO AMYLOID DEPOSITION [J].
BARROW, CJ ;
ZAGORSKI, MG .
SCIENCE, 1991, 253 (5016) :179-182
[2]  
Bernai J. D., 1965, The origins of pre-biological systems and of their molecular matrices., P65
[3]   BETA-STRUCTURES OF ALTERNATING POLYPEPTIDES AND THEIR POSSIBLE PREBIOTIC SIGNIFICANCE [J].
BRACK, A ;
ORGEL, LE .
NATURE, 1975, 256 (5516) :383-387
[4]   CONSERVATION OF INFECTIVITY IN PURIFIED FIBRILLARY EXTRACTS OF SCRAPIE-INFECTED HAMSTER BRAIN AFTER SEQUENTIAL ENZYMATIC DIGESTION OR POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].
BROWN, P ;
LIBERSKI, PP ;
WOLFF, A ;
GAJDUSEK, DC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (18) :7240-7244
[5]   ENDOGENOUS PRODUCTION, EXOGENOUS DELIVERY AND IMPACT-SHOCK SYNTHESIS OF ORGANIC-MOLECULES - AN INVENTORY FOR THE ORIGINS OF LIFE [J].
CHYBA, C ;
SAGAN, C .
NATURE, 1992, 355 (6356) :125-132
[6]   MOLECULAR DETERMINANTS OF AMYLOID DEPOSITION IN ALZHEIMERS-DISEASE - CONFORMATIONAL STUDIES OF SYNTHETIC BETA-PROTEIN FRAGMENTS [J].
HALVERSON, K ;
FRASER, PE ;
KIRSCHNER, DA ;
LANSBURY, PT .
BIOCHEMISTRY, 1990, 29 (11) :2639-2644
[7]   AGGREGATION AND SECONDARY STRUCTURE OF SYNTHETIC AMYLOID BETA-A4 PEPTIDES OF ALZHEIMERS-DISEASE [J].
HILBICH, C ;
KISTERSWOIKE, B ;
REED, J ;
MASTERS, CL ;
BEYREUTHER, K .
JOURNAL OF MOLECULAR BIOLOGY, 1991, 218 (01) :149-163
[8]  
IQBAL K, 1983, ALZHEIMERS DISEASE S, P48
[9]   CRYSTAL-STRUCTURE OF 4-STRANDED OXYTRICHA TELOMERIC DNA [J].
KANG, C ;
ZHANG, XH ;
RATLIFF, R ;
MOYZIS, R ;
RICH, A .
NATURE, 1992, 356 (6365) :126-131
[10]   SYNTHETIC PEPTIDE HOMOLOGOUS TO BETA-PROTEIN FROM ALZHEIMER-DISEASE FORMS AMYLOID-LIKE FIBRILS INVITRO [J].
KIRSCHNER, DA ;
INOUYE, H ;
DUFFY, LK ;
SINCLAIR, A ;
LIND, M ;
SELKOE, DJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (19) :6953-6957
123