THE TDH AND SERA OPERONS OF ESCHERICHIA-COLI - MUTATIONAL ANALYSIS OF THE REGULATORY ELEMENTS OF LEUCINE-RESPONSIVE GENES

被引:58
作者
REX, JH [1 ]
ARONSON, BD [1 ]
SOMERVILLE, RL [1 ]
机构
[1] PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907
关键词
D O I
10.1128/jb.173.19.5944-5953.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The tdh promoter of Escherichia coli is induced seven- to eightfold when cells are grown in the presence of exogenous leucine. A scheme was devised to select mutants that exhibited high constitutive expression of the tdh promoter. The mutations in these strains were shown to lie within a previously identified gene (lrp) that encodes Lrp (leucine-responsive regulatory protein). By deletion analysis, the site of action of Lrp was localized to a 25-bp region between coordinates -69 and -44 of the tdh promoter. Disruption of a 12-bp presumptive target sequence found in this region of tdh resulted in constitutively derepressed expression from the tdh promoter. Similar DNA segments (consensus, TTTATTCtNaAT) were also identified in a number of other promoters, including each of the Lrp-regulated promoters whose nucleotide sequence is known. The sequence of the promoter region of serA, an Lrp-regulated gene, was determined. No Lrp consensus target sequence was present upstream of serA, suggesting that Lrp acts indirectly on the serA promoter. A previously described mutation in a leucine-responsive trans-acting factor, LivR (J. J. Anderson, S. C. Quay, and D. L. Oxender, J. Bacteriol. 126:80-90, 1976), resulted in constitutively repressed expression from the tdh promoter and constitutively induced expression from the serA promoter. The possibility that LivR and Lrp are allelic is discussed.
引用
收藏
页码:5944 / 5953
页数:10
相关论文
共 51 条
[11]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[12]   SITE-DIRECTED MUTAGENESIS OF THE ESCHERICHIA-COLI CHROMOSOME NEAR ORIC - IDENTIFICATION AND CHARACTERIZATION OF ASNC, A REGULATORY ELEMENT IN ESCHERICHIA-COLI ASPARAGINE METABOLISM [J].
DEWIND, N ;
DEJONG, M ;
MEIJER, M ;
STUITJE, AR .
NUCLEIC ACIDS RESEARCH, 1985, 13 (24) :8797-8811
[13]   DERIVATION OF GLYCINE FROM THREONINE IN ESCHERICHIA-COLI K-12 MUTANTS [J].
FRASER, J ;
NEWMAN, EB .
JOURNAL OF BACTERIOLOGY, 1975, 122 (03) :810-817
[14]   INTEGRATION-NEGATIVE MUTANTS OF BACTERIOPHAGE LAMBDA [J].
GOTTESMAN, ME ;
YARMOLINSKY, MB .
JOURNAL OF MOLECULAR BIOLOGY, 1968, 31 (03) :487-+
[15]   ROLE OF METHIONINE IN REGULATION OF SERINE HYDROXYMETHYLTRANSFERASE IN ESCHERICHIA-COLI [J].
GREENE, RC ;
RADOVICH, C .
JOURNAL OF BACTERIOLOGY, 1975, 124 (01) :269-278
[16]   CHARACTERIZATION OF A NOVEL L-SERINE TRANSPORT-SYSTEM IN ESCHERICHIA-COLI [J].
HAMA, H ;
SHIMAMOTO, T ;
TSUDA, M ;
TSUCHIYA, T .
JOURNAL OF BACTERIOLOGY, 1988, 170 (05) :2236-2239
[17]   TRANSPORT OF L-4-AZALEUCINE IN ESCHERICHIA-COLI [J].
HARRISON, LI ;
CHRISTENSEN, HN ;
HANDLOGTEN, ME ;
OXENDER, DL ;
QUAY, SC .
JOURNAL OF BACTERIOLOGY, 1975, 122 (03) :957-965
[18]   METABOLITES INFLUENCE CONTROL OF LYSINE TRANSFER RIBONUCLEIC-ACID SYNTHETASE FORMATION IN ESCHERICHIA-COLI K-12 [J].
HIRSHFIELD, IN ;
YEH, FM ;
SAWYER, LE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (04) :1364-1367
[19]   RAPID AND EFFICIENT COSMID CLONING [J].
ISHHOROWICZ, D ;
BURKE, JF .
NUCLEIC ACIDS RESEARCH, 1981, 9 (13) :2989-2998