APPLICATION OF MATRIX SOLID-PHASE DISPERSION FOR THE DETERMINATION OF CLENBUTEROL IN LIVER SAMPLES

A rapid method for determination of clenbuterol in liver samples has been developed. It involves combining matrix solid phase dispersion with radioimmunoassay as an efficient technique for residue determination at the sub ng g-1 level. The technique was optimised for extract clean-up and recovery of residue using radiolabelled [H-3]-clenbuterol. Inter- and intra-assay validation, carried out on fortified liver samples, showed high recoveries over the range 1-5 ng clenbuterol g-1. The determination of incurred residues in liver samples has been investigated. The developed procedure allows for determination of residues of clenbuterol in tissues at levels of less than 0.5 ng g-1.