NEW VIRUS-SPECIFIC T-HELPER EPITOPES OF FOOT-AND-MOUTH-DISEASE VIRAL VP(1) PROTEIN

被引：13

作者：

VOLPINA, OM

GELFANOV, VM

YAROV, AV

SUROVOY, AY

CHEPURKIN, AV

IVANOV, VT

机构：

[1] Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117871 GSP Moscow, Miklukho-Maklaya

来源：

FEBS LETTERS | 1993年 / 333卷 / 1-2期

关键词：

FOOT-AND-MOUTH DISEASE; SYNTHETIC PEPTIDE IMMUNOGENICITY; T-CELL RECOGNITION SITE;

D O I：

10.1016/0014-5793(93)80399-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immunogenicity studies of synthetic peptides from different regions of VP1 protein of foot-and-mouth disease virus strain A22 revealed the following active fragments: 39-61, 50-69, 135-159, 175-189, 170-189 and 197-213. Testing of virus neutralizing antibody production in rabbits primed by peptides and then inoculated by the virus showed that only peptides 135-159 and 170-189 were able to induce the functional T-cell helper activity. Localization of virus-specific T-cell recognition sites in sequences 135-159 and 170-189 was confirmed in in vitro recognition experiments of the virus by peptide activated mice lymphocytes.

引用

页码：175 / 178

页数：4